Synchrotron X-ray fluorescence studies of a bromine-labelled cyclic RGD peptide interacting with individual tumor cells
نویسندگان
چکیده
The first example of synchrotron X-ray fluorescence imaging of cultured mammalian cells in cyclic peptide research is reported. The study reports the first quantitative analysis of the incorporation of a bromine-labelled cyclic RGD peptide and its effects on the biodistribution of endogenous elements (for example, K and Cl) within individual tumor cells.
منابع مشابه
Construction of expressing vectors including melanoma differentiation-associated gene-7 (mda-7) fused with the RGD sequences for better tumor targeting
Objective(s): Up to now, many researches have been performed to improve the antitumoral effect of melanoma differentiation-associated gene-7 (mda-7) protein. The purpose of our research was to construct 3 expression vectors producing mda-7 in fusion with RGD (Arginine-Glycine-Aspartic acid) peptide and evaluate their expression. Materials and Methods: mda-7 gene with two different RGD sequ...
متن کاملBinding and Uptake of RGD-Containing Ligands to Cellular avb3 Integrins
The cyclic peptide, cRGDf[N(me)]V, binds to the avb3 integrin and can disrupt binding of the integrin to its natural ligands in the extracellular matrix. In this work, the ability of a water-soluble, fluorescently labeled variant of the RGD-containing peptide (cRGDfK-488) to bind to integrins on human umbilical vascular endothelial cells (HUVEC) and subsequently undergo endocytosis was characte...
متن کاملTumor targeting and imaging with dual-peptide conjugated multifunctional liposomal nanoparticles
BACKGROUND The significant progress in nanotechnology provides a wide spectrum of nanosized material for various applications, including tumor targeting and molecular imaging. The aim of this study was to evaluate multifunctional liposomal nanoparticles for targeting approaches and detection of tumors using different imaging modalities. The concept of dual-targeting was tested in vitro and in v...
متن کاملPreparation and Bioevaluation of DOTA-Cyclic RGD Peptide Dimer Labeled with 68Ga
Radiolabeled cyclic RGD peptides targeting integrin αvβ3 are reported as promising agents for the early diagnosis of metastatic tumors. With an aim to improve tumor uptake and retention of the peptide, cyclic RGD peptide dimer E[c (RGDfK)] 2 (E = Glutamic acid, f = phenyl alanine, K = lysine) coupled to the bifunctional chelator DOTA was custom synthesized and radiolabelled with Ga. Radiolabell...
متن کاملA Review of the Applications of Synchrotron Radiation in Archaeological Sciences
The scientific research regarding investigation, characterization and protection of the archeological specimens is manifested through a notable participation of multidisciplinary subjects and experts, scientists and archeometrists. One of the main principals which are considered by archaeometrists in the study of the precious specimens is the utilizing nondestructive methods. As an example, in ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 20 شماره
صفحات -
تاریخ انتشار 2013